Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.00027085
Body weight after experiment	0.04912711
Oxygen consumption	0.00152635
Carbon dioxide production	0.00072255
Heat production (metabolic rate)	0.17769476
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.03441749
Cumulative food intake	0.01927908
Respiratory Exchange Ratio	0.03192387
Total water intake	0.34584274
Cumulative water intake	0.17769476
Respiratory Exchange Ratio series	0.01611551
XTOT	0.46039666
XAMB	0.16568550
YTOT	0.09358115
YAMB	0.03740908
ZTOT	0.53007100
ZAMB	0.34546492
Oxygen consumption series	0.00245191
Carbon dioxide production series	0.00039795
Heat production (metabolic rate) series	0.20131687
Total food intake series	0.03928273
Total water intake series	0.10660980
XTOT series	0.26946118
XAMB series	0.07713773
YTOT series	0.07677033
YAMB series	0.02067457
ZTOT series	0.36081817
ZAMB series	0.26160076