Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.46640632
Body weight after experiment	0.16565517
Oxygen consumption	-
Carbon dioxide production	-
Heat production (metabolic rate)	-
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.32691167
Cumulative food intake	0.53895130
Respiratory Exchange Ratio	-
Total water intake	0.55785012
Cumulative water intake	0.74150911
Respiratory Exchange Ratio series	-
XTOT	0.79702534
XAMB	0.95338679
YTOT	0.89107032
YAMB	0.81076574
ZTOT	0.08859458
ZAMB	0.37543058
Oxygen consumption series	-
Carbon dioxide production series	-
Heat production (metabolic rate) series	-
Total food intake series	0.83671351
Total water intake series	0.98645174
XTOT series	0.69389625
XAMB series	0.98903491
YTOT series	0.85908172
YAMB series	0.81223185
ZTOT series	0.30525342
ZAMB series	0.47072061