Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.03588131
Body weight after experiment	0.11151394
Oxygen consumption	0.56123061
Carbon dioxide production	0.07853260
Heat production (metabolic rate)	0.73771656
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.15953897
Cumulative food intake	0.40821871
Respiratory Exchange Ratio	0.00072255
Total water intake	0.04833114
Cumulative water intake	0.12498113
Respiratory Exchange Ratio series	0.00018385
XTOT	0.00724093
XAMB	0.01020963
YTOT	0.01102437
YAMB	0.02674477
ZTOT	0.37937054
ZAMB	0.27520827
Oxygen consumption series	0.49022495
Carbon dioxide production series	0.06374321
Heat production (metabolic rate) series	0.87532892
Total food intake series	0.82899887
Total water intake series	0.23169278
XTOT series	0.00005785
XAMB series	0.00014349
YTOT series	0.00233086
YAMB series	0.00826987
ZTOT series	0.43801682
ZAMB series	0.42428528