Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.76829268
Body weight after experiment	0.14623320
Oxygen consumption	0.01687776
Carbon dioxide production	0.00192079
Heat production (metabolic rate)	0.00503776
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.00011768
Cumulative food intake	0.00010044
Respiratory Exchange Ratio	0.00067135
Total water intake	0.00084668
Cumulative water intake	0.00112717
Respiratory Exchange Ratio series	0.00017708
XTOT	0.05270363
XAMB	0.15572313
YTOT	0.17399480
YAMB	0.28014934
ZTOT	0.26839155
ZAMB	0.26934321
Oxygen consumption series	0.00466653
Carbon dioxide production series	0.00005204
Heat production (metabolic rate) series	0.00067041
Total food intake series	0.00105979
Total water intake series	0.00183703
XTOT series	0.07790528
XAMB series	0.23181058
YTOT series	0.25103303
YAMB series	0.47003727
ZTOT series	0.73989982
ZAMB series	0.74730728