Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.43057484
Body weight after experiment	0.04734612
Oxygen consumption	0.09097829
Carbon dioxide production	0.02708057
Heat production (metabolic rate)	0.08152577
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	-
Cumulative food intake	-
Respiratory Exchange Ratio	0.01828959
Total water intake	0.00232796
Cumulative water intake	0.00179436
Respiratory Exchange Ratio series	0.01015480
XTOT	0.46839598
XAMB	0.10855755
YTOT	0.55759822
YAMB	0.17115731
ZTOT	0.75516665
ZAMB	0.64695146
Oxygen consumption series	0.02382431
Carbon dioxide production series	0.00265308
Heat production (metabolic rate) series	0.02458060
Total food intake series	-
Total water intake series	0.01926485
XTOT series	0.59675646
XAMB series	0.14166307
YTOT series	0.62819147
YAMB series	0.19101084
ZTOT series	0.53218170
ZAMB series	0.44335346