Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.15114227
Body weight after experiment	0.02043313
Oxygen consumption	0.00068093
Carbon dioxide production	0.00166406
Heat production (metabolic rate)	0.00337048
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.94499852
Cumulative food intake	0.56740263
Respiratory Exchange Ratio	0.70841028
Total water intake	0.24870169
Cumulative water intake	0.95523330
Respiratory Exchange Ratio series	0.66232991
XTOT	0.19097403
XAMB	0.12515749
YTOT	0.55597690
YAMB	0.43831491
ZTOT	0.57331711
ZAMB	0.36979653
Oxygen consumption series	0.00000387
Carbon dioxide production series	0.00000544
Heat production (metabolic rate) series	0.00012670
Total food intake series	0.46893597
Total water intake series	0.18824005
XTOT series	0.12099376
XAMB series	0.09899594
YTOT series	0.40719625
YAMB series	0.35176833
ZTOT series	0.67233508
ZAMB series	0.71528286