Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.03918278
Body weight after experiment	0.40998662
Oxygen consumption	0.00030033
Carbon dioxide production	0.00012095
Heat production (metabolic rate)	0.00119550
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.09126551
Cumulative food intake	0.00101308
Respiratory Exchange Ratio	0.13648848
Total water intake	0.89608960
Cumulative water intake	0.08279894
Respiratory Exchange Ratio series	0.13397576
XTOT	0.83332790
XAMB	0.88315513
YTOT	0.77135634
YAMB	0.98530513
ZTOT	0.75165307
ZAMB	0.69445770
Oxygen consumption series	0.00024998
Carbon dioxide production series	0.00008619
Heat production (metabolic rate) series	0.00320298
Total food intake series	0.09923668
Total water intake series	0.42405790
XTOT series	0.84296723
XAMB series	0.94569441
YTOT series	0.79228199
YAMB series	0.87018784
ZTOT series	0.47035403
ZAMB series	0.42754394