Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.39199641
Body weight after experiment	0.80292124
Oxygen consumption	0.09307083
Carbon dioxide production	0.97053992
Heat production (metabolic rate)	0.08734802
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.02635750
Cumulative food intake	0.01809874
Respiratory Exchange Ratio	0.00015036
Total water intake	0.00935887
Cumulative water intake	0.00072328
Respiratory Exchange Ratio series	0.00001091
XTOT	0.35483719
XAMB	0.48094005
YTOT	0.55213537
YAMB	0.73393039
ZTOT	0.47256315
ZAMB	0.70191200
Oxygen consumption series	0.15209260
Carbon dioxide production series	0.93142951
Heat production (metabolic rate) series	0.13058816
Total food intake series	0.67742345
Total water intake series	0.11557844
XTOT series	0.35344803
XAMB series	0.60441338
YTOT series	0.58545204
YAMB series	0.86383960
ZTOT series	0.83457961
ZAMB series	0.97202082