Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.01598552
Body weight after experiment	0.02793115
Oxygen consumption	0.59905524
Carbon dioxide production	0.46013627
Heat production (metabolic rate)	0.28014934
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.30425252
Cumulative food intake	0.28795923
Respiratory Exchange Ratio	0.75869129
Total water intake	0.49134208
Cumulative water intake	0.92795564
Respiratory Exchange Ratio series	0.70963452
XTOT	0.77647114
XAMB	0.66897791
YTOT	0.74846549
YAMB	0.81951677
ZTOT	0.21953960
ZAMB	0.28985780
Oxygen consumption series	0.51326463
Carbon dioxide production series	0.48377296
Heat production (metabolic rate) series	0.37582697
Total food intake series	0.76840711
Total water intake series	0.41815598
XTOT series	0.86395946
XAMB series	0.91592351
YTOT series	0.72841384
YAMB series	0.66965153
ZTOT series	0.68079212
ZAMB series	0.30185409