Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.82512618
Body weight after experiment	0.76352932
Oxygen consumption	0.00120962
Carbon dioxide production	0.07246342
Heat production (metabolic rate)	0.00145746
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.05608864
Cumulative food intake	0.00316611
Respiratory Exchange Ratio	0.00005850
Total water intake	0.25139829
Cumulative water intake	0.00011208
Respiratory Exchange Ratio series	0.00000019
XTOT	0.76115671
XAMB	0.97069018
YTOT	0.58153757
YAMB	0.89741437
ZTOT	0.50408056
ZAMB	0.22938669
Oxygen consumption series	0.00096112
Carbon dioxide production series	0.07015331
Heat production (metabolic rate) series	0.00159464
Total food intake series	0.79211840
Total water intake series	0.71257084
XTOT series	0.85191788
XAMB series	0.81628650
YTOT series	0.62822933
YAMB series	0.93773825
ZTOT series	0.78858896
ZAMB series	0.44492028