Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.08569601
Body weight after experiment	0.12022762
Oxygen consumption	0.27569980
Carbon dioxide production	0.58190141
Heat production (metabolic rate)	0.98129809
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.07824803
Cumulative food intake	0.49340420
Respiratory Exchange Ratio	0.30901806
Total water intake	0.24116671
Cumulative water intake	0.02057539
Respiratory Exchange Ratio series	0.28232321
XTOT	0.98667498
XAMB	0.79187297
YTOT	0.70584105
YAMB	0.88682451
ZTOT	0.47462999
ZAMB	0.42425839
Oxygen consumption series	0.42810714
Carbon dioxide production series	0.66770195
Heat production (metabolic rate) series	0.80279335
Total food intake series	0.79707942
Total water intake series	0.80742559
XTOT series	0.92361786
XAMB series	0.98634923
YTOT series	0.78367234
YAMB series	0.75615661
ZTOT series	0.89850559
ZAMB series	0.71411986