Procedure Metadata:

Procedure Introduction:

Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through indirect calorimetry by measuring oxygen consumption with an open flow respirometric system. CO2 and O2 sensors measure the difference in CO2 and O2 concentrations in air volumes flowing through control or animal cages. The amount of oxygen consumed over a given period of time can thus be calculated, as far as the air flow through the cage is known. Data are expressed as ml O2 h-1animal-1. The system also monitors CO2 production, therefore, the respiratory exchange ratio (RER) and heat production can be calculated. An activity and food and water intake monitoring system can also be integrated into the set up in order to investigate circadian pattern and behavior.

Procedure Parameters:

Parameter Name	Male P-Value	Detail
Body weight before experiment	0.01763289
Body weight after experiment	0.49629556
Oxygen consumption	0.34584274
Carbon dioxide production	0.92643759
Heat production (metabolic rate)	0.00705791
Ambulatory activity (no. of beam cuts)	-
Total activity (no. of fine movement + no. of beam cuts)	-
Total food intake	0.00560848
Cumulative food intake	0.00095433
Respiratory Exchange Ratio	0.00856806
Total water intake	0.00365208
Cumulative water intake	0.02539664
Respiratory Exchange Ratio series	0.00518230
XTOT	0.05823587
XAMB	0.22917462
YTOT	0.35483719
YAMB	0.73140986
ZTOT	0.00895602
ZAMB	0.00207897
Oxygen consumption series	0.48729361
Carbon dioxide production series	0.87783076
Heat production (metabolic rate) series	0.01803948
Total food intake series	0.41016040
Total water intake series	0.05752959
XTOT series	0.01634999
XAMB series	0.07431004
YTOT series	0.17711698
YAMB series	0.43181241
ZTOT series	0.08599263
ZAMB series	0.00989727